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Image Search Results


The mass spectral data of the bioactive PRAS40 binding metabolites using HPLC-MSESI-QTOF and MS/MS in positive mode.

Journal: Frontiers in Pharmacology

Article Title: Screening and evaluation of metabolites binding PRAS40 from Erxian decoction used to treat spinal cord injury

doi: 10.3389/fphar.2024.1339956

Figure Lengend Snippet: The mass spectral data of the bioactive PRAS40 binding metabolites using HPLC-MSESI-QTOF and MS/MS in positive mode.

Article Snippet: For reference, Indole (No. I104724 ) and palmatine chloride (No. P274975) were acquired from Aladdin Co., Ltd (Shanghai, China).

Techniques: Binding Assay, Concentration Assay

ITC traces and binding isotherms between the ligands and PRAS40. (A) Palmatine and PRAS40. (B) Indole and PRAS40.

Journal: Frontiers in Pharmacology

Article Title: Screening and evaluation of metabolites binding PRAS40 from Erxian decoction used to treat spinal cord injury

doi: 10.3389/fphar.2024.1339956

Figure Lengend Snippet: ITC traces and binding isotherms between the ligands and PRAS40. (A) Palmatine and PRAS40. (B) Indole and PRAS40.

Article Snippet: For reference, Indole (No. I104724 ) and palmatine chloride (No. P274975) were acquired from Aladdin Co., Ltd (Shanghai, China).

Techniques: Binding Assay

The ITC data of palmatine and indole binding with PRAS40.

Journal: Frontiers in Pharmacology

Article Title: Screening and evaluation of metabolites binding PRAS40 from Erxian decoction used to treat spinal cord injury

doi: 10.3389/fphar.2024.1339956

Figure Lengend Snippet: The ITC data of palmatine and indole binding with PRAS40.

Article Snippet: For reference, Indole (No. I104724 ) and palmatine chloride (No. P274975) were acquired from Aladdin Co., Ltd (Shanghai, China).

Techniques: Binding Assay

Molecular docking. (A) 2D structure of palmatine. (B) 2D structure of indole. (C) 3D structure of PRAS40. (D) Docking of PRAS40 to palmatine in 3D view, and (E) 2D view. (F) Docking of PRAS40 to indole in 3D view, and (G) 2D view.

Journal: Frontiers in Pharmacology

Article Title: Screening and evaluation of metabolites binding PRAS40 from Erxian decoction used to treat spinal cord injury

doi: 10.3389/fphar.2024.1339956

Figure Lengend Snippet: Molecular docking. (A) 2D structure of palmatine. (B) 2D structure of indole. (C) 3D structure of PRAS40. (D) Docking of PRAS40 to palmatine in 3D view, and (E) 2D view. (F) Docking of PRAS40 to indole in 3D view, and (G) 2D view.

Article Snippet: For reference, Indole (No. I104724 ) and palmatine chloride (No. P274975) were acquired from Aladdin Co., Ltd (Shanghai, China).

Techniques:

Palmatine activates autophagic flux by PRAS40/mTOR pathway. (A) Western blot analysis for determining the expression of p-mTOR, mTOR, p-PRAS40, PRAS40, and LC3 (n = 3) (B) Quantification of the ratio of LC3-Ⅱ to LC3-Ⅰ(∗∗ p < 0.01), (C) p-PRAS40 to PRAS40 (∗ p < 0.01), and (D) p-mTOR to mTOR(∗ p < 0.01, ∗∗∗∗ p < 0.001). The data are depicted as the mean ± SEM.

Journal: Frontiers in Pharmacology

Article Title: Screening and evaluation of metabolites binding PRAS40 from Erxian decoction used to treat spinal cord injury

doi: 10.3389/fphar.2024.1339956

Figure Lengend Snippet: Palmatine activates autophagic flux by PRAS40/mTOR pathway. (A) Western blot analysis for determining the expression of p-mTOR, mTOR, p-PRAS40, PRAS40, and LC3 (n = 3) (B) Quantification of the ratio of LC3-Ⅱ to LC3-Ⅰ(∗∗ p < 0.01), (C) p-PRAS40 to PRAS40 (∗ p < 0.01), and (D) p-mTOR to mTOR(∗ p < 0.01, ∗∗∗∗ p < 0.001). The data are depicted as the mean ± SEM.

Article Snippet: For reference, Indole (No. I104724 ) and palmatine chloride (No. P274975) were acquired from Aladdin Co., Ltd (Shanghai, China).

Techniques: Western Blot, Expressing

Free radical-scavenging capacities of antioxidant activity available measured with DPPH and ABTS assay on microwell plate.

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Antioxidant and Anti-Inflammatory Activity Determination of One Hundred Kinds of Pure Chemical Compounds Using Offline and Online Screening HPLC Assay

doi: 10.1155/2015/165457

Figure Lengend Snippet: Free radical-scavenging capacities of antioxidant activity available measured with DPPH and ABTS assay on microwell plate.

Article Snippet: 74 , Palmatine chloride hydrate , Sigma , 257.82 , 2.57 ± 2.42 , 84.15 ± 2.43.

Techniques: Antioxidant Activity Assay, ABTS Assay, Concentration Assay